Saturday, April 25, 2020

Unknown Lab Report Essay Example

Unknown Lab Report Paper Results Unknown #25 had the following morphology on a streak plate: medium sized buyouts cream colored colony. Grandstanding was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that t was a Gram negative single bacillus, biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test purpose I Reagents Observations I Results I Gram stain I To determine the Gram reaction tooth bacterium Crystal violet, Iodine, Alcohol, Sarnia Pink rod Cram-negative rods I Carbohydrate (Glucose) fermentation To determine the ability of a bacterium to ferment a carbohydrate, make acid or gas Durham tube Color change from red to yellow, bubble in Durham tube Positive carbohydrate fermentation, acid reduction and gas production Thyrotrophic digestion I To determine the ability of an organism to split indolence from thyrotrophic I Kavas drop added to Trenton broth I Kind of colorless and list green ring at top of broth Negative thyrotrophic digestion Sistine digestion I To determine the ability of an organism to catabolic certain amino acids I Iron ions I Color didnt changed remained creamy Negative Sistine digestion Citrate Utilization To determine the organism is able to utilize citrate as a carbon source I Citrate slant (green) Biorhythms blue I Color changed from green to deep blue I Positive for titrate utilization urea digestion To determine the ability of an organism to catabolic ammonia Phenol Color didnt change stayed bright orange Negative for urea digestion I Flowchart Gram stain Gram-negative Rod Glucose fermentation (positive) Positive Unknown# 25 Negative Escherichia coli Pseudonymous organisms Interrogator arrogates Pseudonymous fluorescent Salmonella enteritis Seriate mercenaries Protests vulgarism Gelatin drowsily (positive) Positive Negative Seriate mercenaries Salmonella enteritis Motility test (negative) Escherichia coli Interrogator arrogates Unknown# 3 Protests vulgarism Discussion/ Conclusion After several differential tests, it was concluded that unknown# 25 was Protests vulgarism. Tater performing the Gram stain to determine that the unknown was a Gram negative rod, the organism grown on slant for use in inoculating the rest of the biochemical tests. All of the biochemical tests worked well except for the motility, cytosine digestion and urea digestion. We will write a custom essay sample on Unknown Lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Unknown Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer According to chart it should be positive but because of the human error, not appropriate temperature, and too old culture, etc it gave a false negative result. For example: the culture that seed it might be too old, or when was inoculating the culture to the urea it might be hot, leaded to kill the bacterium _ That is Why got false negative result. Other than this everything (data) was great and into the conclusion the unknown was Protests vulgarism. P. vulgarism is in the Intercontinental family and opportunistic pathogen of humans. Colonies of Protests inhabits the intestinal tracts Of humans and animals. It can be found in soil, water and fecal matter.